OVERVIEW

Simple Western™ platforms are the only systems on the market that fully automate traditional western blotting, providing data on 24 samples in only 3 hours or 96 samples overnight. Separate and analyze proteins by size from 2-440 kDa either by immunoassay or total protein analysis. There's even an automated charge-based assay utilizing isoelectric focusing if you're doing post-translational modification characterization. You'll get quantitative results, reproducibility that's spot on, and use less sample in the process. So, what's the big difference between Simple Western automated western blot systems and traditional western blot tests? Our Simple Western instruments are the only gel-free, blot-free, and hands-free capillary-based immunoassay platforms that integrate and automate the entire protein separation and detection process. So you'll have more time to get down to real science.

Bringing Traditional Western Blotting Into the 21st Century

Historically, the traditional Western blot has been widely used in the study of protein biochemistry, but it is notoriously labor-intensive, and limited in reproducibility and quantification, among other challenges. Developed in the 1970s, the original Western blotting technique has remained virtually the same with limited improvements made to meet the demands of researchers.

Simple Western completely eliminates the manual and time-consuming steps of traditional western blotting workflows including the long and variable transfer step and the manual incubation, washing and detection steps that lead to the high variability and poor reproducibility that has plagued traditional Western blotting workflows for years.

Simple Western’s hands-free automation, rapid time to results, quantitation, high sensitivity and reproducibility make it the technology of choice to meet researcher’s Western blot analysis needs in the 21st Century.

What Is the Difference Between Simple Western Automated Western Blot Systems and Traditional Western Blot Workflows?

Simple Western platforms redefine your western blotting workflow by fully automating all assay steps including separation, immunodetection and data analysis. Simple Western platforms are based on capillary electrophoresis and Simple Western assays are performed in capillaries where SDS/heat-denatured proteins are separated by apparent molecular weight, immobilized covalently via a UV sensitive coating in the capillary, detected directly in the capillary by primary and secondary antibodies and analyzed by the integrated Compass Software.

By integrating and automating these previously manual workflow steps, Simple Western platforms overcome many of the limitations associated with traditional Western blotting workflows, significantly reducing hands-on time, and providing excellent reproducibility and assay transferability. Simple Western assays are quantitative, enabling generation of full dose response curves, and also include built-in total protein normalization so you can easily and accurately normalize your immunoassay data.

Simple Western is easy to use and also offers higher throughput and faster time to results. With only 30 min set-up times and walk away automation, you can get fully analyzed western blotting data on 25 samples in less than 3 hours on Jess, Abby, and Wes. If you want even higher throughput, Sally Sue or Peggy Sue can analyze up to 96 samples in a single run!

With all these advantages, Simple Western is rapidly becoming accepted as the preferred replacement for traditional Western blot protocols.

Simple Western System	Jess	Abby	Wes	Sally Sue	Peggy Sue	Traditional Westerns
Maximum samples per run	25	25	25	96	96	25
Run time for mass samples (size assays)	<3 hours	<3 hours	<3 hours	<14 - 19 hours	<11 - 19 hours	2 days
Hands-on time	30 minutes	30 minutes	30 minutes	1 hour	1 hour	3.25 hours

 

Simple Western – Your Partner for Publication Perfect Data!

Increasingly, the scientific community is setting new and more rigorous standards for data reproducibility, transparency and rigor in grant submission guidelines and journal publications. With over a thousand publications and counting, Simple Western systems overcome the challenges associated with traditional western blotting workflows to produce publication-ready data that meet the highest standards of data integrity, reproducibility and rigor.

SIMPLE WESTERN ASSAY: HOW DOES IT WORK?

Key Benefits of Using Simple Western for Publication-ready Data:

imple Western System	Jess	Abby	Wes	Sally Sue	Peggy Sue	NanoPro 1000
Simple Western size assays
Simple Western charge assays
Chemiluminescence
Fluorescence
Maximum samples per run	25	25	25	96	96	96
Run time for max samples (size)	< 3 hours	< 3 hours	< 3 hours	14-19 hours	11-19 hours	N/A
Run time for max samples (charge)	N/A	N/A	N/A	N/A	11 hours	11 hours
Sample cooling (size)	N/A	N/A	N/A	10°C	10°C	N/A
Sample cooling (charge)	N/A	N/A	N/A	N/A	3°C	3°C
Operating humidity range	20-60% relative, non-condensing
Operating temp range	18-24°C
Power	100-230 V AC, 50/60 Hz
Dimensions (H x W x D)	33 x 33 x 52 cm	33 x 33 x 52 cm	33 x 33 x 52 cm	84 x 94 x 61 cm	84 x 94 x 61 cm	84 x 94 x 61 cm
Part number	004-650	004-680	004-600	004-700	004-800	004-109

	Immunoassay Size Specification		Immunoassay Charge Specification
Description	Chemiluminescence	Fluorescence
Sample required	0.3-1.2 µg lysate	0.6-1.2 µg	0.6-1.2 µg
Size or pI range	2-40, 12-230 and 66-440 kDa	2-40, 12-230 and 66-440 kDa	Widest gradient ranges from pI 3 to pI 10
Sizing CV	<10%	<10%	<10%
Intra-assay CV	<15%	<15%	<20%
Inter-assay CV	<20%*	<20%*	<20%**
Resolution (± percent difference in MW)	± 15-20% for MW < 20 kDa ± 10% for MW > 20 kDa	± 15-20% for MW < 20 kDa ± 10% for MW > 20 kDa	± 0.1 pI units
Quantitation CV	<20%	<20%	<20%
Dynamic range	up to 4 logs***	3-4 logs	3 logs
Sensitivity	Low pg	High pg	Low pg
Capillary	5 cm, 100 µm, 400 nL