Automated Western System

OVERVIEW

Simple Western™ platforms are the only systems on the market that fully automate traditional western blotting, providing data on 24 samples in only 3 hours or 96 samples overnight. Separate and analyze proteins by size from 2-440 kDa either by immunoassay or total protein analysis. There's even an automated charge-based assay utilizing isoelectric focusing if you're doing post-translational modification characterization. You'll get quantitative results, reproducibility that's spot on, and use less sample in the process. So, what's the big difference between Simple Western automated western blot systems and traditional western blot tests? Our Simple Western instruments are the only gel-free, blot-free, and hands-free capillary-based immunoassay platforms that integrate and automate the entire protein separation and detection process. So you'll have more time to get down to real science.

Bringing Traditional Western Blotting Into the 21st Century


Historically, the traditional Western blot has been widely used in the study of protein biochemistry, but it is notoriously labor-intensive, and limited in reproducibility and quantification, among other challenges. Developed in the 1970s, the original Western blotting technique has remained virtually the same with limited improvements made to meet the demands of researchers.

Simple Western completely eliminates the manual and time-consuming steps of traditional western blotting workflows including the long and variable transfer step and the manual incubation, washing and detection steps that lead to the high variability and poor reproducibility that has plagued traditional Western blotting workflows for years.

Simple Western’s hands-free automation, rapid time to results, quantitation, high sensitivity and reproducibility make it the technology of choice to meet researcher’s Western blot analysis needs in the 21st Century.


What Is the Difference Between Simple Western Automated Western Blot Systems and Traditional Western Blot Workflows?


Simple Western platforms redefine your western blotting workflow by fully automating all assay steps including separation, immunodetection and data analysis. Simple Western platforms are based on capillary electrophoresis and Simple Western assays are performed in capillaries where SDS/heat-denatured proteins are separated by apparent molecular weight, immobilized covalently via a UV sensitive coating in the capillary, detected directly in the capillary by primary and secondary antibodies and analyzed by the integrated Compass Software.


By integrating and automating these previously manual workflow steps, Simple Western platforms overcome many of the limitations associated with traditional Western blotting workflows, significantly reducing hands-on time, and providing excellent reproducibility and assay transferability. Simple Western assays are quantitative, enabling generation of full dose response curves, and also include built-in total protein normalization so you can easily and accurately normalize your immunoassay data.

Simple Western is easy to use and also offers higher throughput and faster time to results. With only 30 min set-up times and walk away automation, you can get fully analyzed western blotting data on 25 samples in less than 3 hours on Jess, Abby, and Wes. If you want even higher throughput, Sally Sue or Peggy Sue can analyze up to 96 samples in a single run!

With all these advantages, Simple Western is rapidly becoming accepted as the preferred replacement for traditional Western blot protocols.

Simple Western System

Jess

Abby

Wes

Sally Sue

Peggy Sue

Traditional Westerns

Maximum samples per run

25

25

25

96

96

25

Run time for mass samples (size assays)

<3 hours

<3 hours

<3 hours

<14 - 19 hours

<11 - 19 hours

2 days

Hands-on time

30 minutes

30 minutes

30 minutes

1 hour

1 hour

3.25 hours

 

Simple Western – Your Partner for Publication Perfect Data!


Increasingly, the scientific community is setting new and more rigorous standards for data reproducibility, transparency and rigor in grant submission guidelines and journal publications. With over a thousand publications and counting, Simple Western systems overcome the challenges associated with traditional western blotting workflows to produce publication-ready data that meet the highest standards of data integrity, reproducibility and rigor.

Key Benefits of Using Simple Western for Publication-ready Data:

  • Automated Simple Western platforms provide reproducible data, making it easy for other labs to reproduce & verify published results.
  • Generate complete electronic data files which include publication-quality images and quantitative results as well as all assay conditions, making it easy to upload to data repositories to meet data sharing requirements.
  • Perform total protein normalization in the same capillary as your immunoassay for more accurate results so you don’t have to rely on variable housekeeping proteins.
  • Perform reliable sequential immunoassays in the same capillary with RePlex™ rather than struggling with variable stripping & re-probing protocols.
  • Optimize your assay within hours making use of the vast selection of Simple Western certified antibodies.
  • Reliably detect low abundance proteins using highly sensitive chemiluminescence or fluorescence detection modes.
  • Get multiplexed western blotting data in the same sample using Jess’ fluorescence capabilities with IR, NIR and chemiluminescence channels.

SIMPLE WESTERN ASSAY: HOW DOES IT WORK?

Key Benefits of Using Simple Western for Publication-ready Data:

imple Western System Jess Abby Wes Sally Sue Peggy Sue NanoPro 1000
Simple Western size assays




 
Simple Western charge assays        

Chemiluminescence





Fluorescence
         
Maximum samples per run 25 25 25 96 96 96
Run time for max samples (size) < 3 hours < 3 hours < 3 hours 14-19 hours 11-19 hours N/A
Run time for max samples (charge) N/A N/A N/A N/A 11 hours 11 hours
Sample cooling (size) N/A N/A N/A 10°C 10°C N/A
Sample cooling (charge) N/A N/A N/A N/A 3°C 3°C
Operating humidity range 20-60% relative, non-condensing
Operating temp range 18-24°C
Power 100-230 V AC, 50/60 Hz
Dimensions (H x W x D) 33 x 33 x 52 cm 33 x 33 x 52 cm 33 x 33 x 52 cm 84 x 94 x 61 cm 84 x 94 x 61 cm 84 x 94 x 61 cm
Part number 004-650 004-680 004-600 004-700 004-800 004-109
  Immunoassay Size Specification Immunoassay Charge Specification
Description Chemiluminescence Fluorescence  
Sample required 0.3-1.2 µg lysate 0.6-1.2 µg 0.6-1.2 µg
Size or pI range 2-40, 12-230 and 66-440 kDa 2-40, 12-230 and 66-440 kDa Widest gradient ranges
from pI 3 to pI 10
Sizing CV <10% <10% <10%
Intra-assay CV <15% <15% <20%
Inter-assay CV <20%* <20%* <20%**
Resolution
(± percent difference in MW)
± 15-20% for MW
< 20 kDa
± 10% for MW
> 20 kDa
± 15-20% for MW
< 20 kDa
± 10% for MW
> 20 kDa
± 0.1 pI units
Quantitation CV <20% <20% <20%
Dynamic range up to 4 logs*** 3-4 logs 3 logs
Sensitivity Low pg High pg Low pg
Capillary 5 cm, 100 µm, 400 nL


SIMPLE WESTERN APPLICATIONS

Cancer & Immuno-Oncology

Cancer & Immuno- Oncology

SARS-CoV-2

SARS-CoV-2

Cell & Gene Therapy

Cell & Gene Therapy

Targeted Degradation

Targeted Degradation

SARS-CoV-2 Serology

SARS-CoV-2 Serology

Exosome

Exosome