Proteome Profiler™ Antibody Arrays

Antibody Array Principle

Proteome Profiler Antibody Arrays are simple and economical multiplex assays for early-stage discovery research.

This semiquantitative antibody array allows for the measurement of more than 100 proteins in a single sample. It requires no specialized equipment and eliminates the need for multiple Western blot experiments. Antibody array kits contain buffers, detection antibodies, and membranes spotted in duplicate with high quality capture antibodies. The arrays utilize chemiluminescence for detection data that can be quantified in the same manner as traditional Western blots.

Select antibody arrays are also suitable for use with the LI-COR® and FluorChem™ detection systems.

 

Antibody Array Principle

  1. Capture antibodies are spotted in duplicate on the nitrocellulose membrane.
  2. Spotted membrane arrays are incubated in a single lysate.
  3. Target analytes are captured by the capture antibody.
  4. Target analytes are detected using a biotinylated or horseradish peroxidase (HRP)-conjugated detection antibody.
  5. Biotinylated detection antibodies are reacted with streptavidin HRP.
  6. Membranes are incubated in a chemiluminescence reagent that produces a light signal proportional to the amount of antibody-target analyte complexes formed.
  7. Data is visualized using standard western blotting methods such as film or digital cameras.

 

Imaging Systems

Proteome Profiler data can be quantified using Fluorchem Imaging Systems. Learn how we quantified TNF-alpha stimulated cytokine secretion in human umbilical vein endothelial cells. See how your peers are using Fluorchem Imagers with antibody arrays.

See the Data and Download the Application Note

Did you know? Proteome Profiler Antibody Arrays are amenable to LI-COR near-infrared fluorescence detection.

 

Quick Spots Image Analysis Software

R&D Systems® now offers Quick Spots image analysis software designed specifically for your Proteome Profiler Arrays.

No more laborious set-up of templates in other imaging software, Quick Spots contains preloaded templates for each Proteome Profiler array. It knows where your results should be and provides the data in less time and with less work.