BaseScope Assays

Push Boundaries with The New Breakthrough Technology for ASO, miRNA, and siRNA Detection

Detections of small non-coding RNAs such as microRNAs and short oligonucleotide therapeutics such as ASO and siRNA require a robust, highly specific and sensitive assay with minimal time and effort, , delivering data for easy interpretation. While microarrays and PCR both provide useful molecular profiles of diseases, important clinically relevant cell and tissue context information is lost along with the spatial variation of gene expression patterns. The miRNAscope™ Assay is a highly sensitive and specific in situ hybridization assay that allows for the visualization of ASO, miRNA, siRNA and other nucleic acid targets between 17-50 nucleotides expression in intact tissues or cultured cells with single-cell resolution, preserving spatial and morphological context.

“Our experience working with ACD Bio has been all-around excellent. With their expertise and through clear communication, we developed several assays to detect both RNA and short oligonucleotides in tissue. Compared to standard complementary probes, the signal we obtained using ACD Bio was evident and specific. After seeing the first images, we immediately knew we wanted to use these techniques more frequently." --Exicure, R&D Team

“We had a great experience using miRNAscope™ in our compound screening efforts. The technique is incredibly specific and the results are very easy to understand. Also, working with ACD’s team has been extremely beneficial. They communicated with us during the process to ensure we can get the answers to our questions. They generated impeccable tissue stainings!” --A leading BioPharma company in NY, USA

miRNAscope HD Assay Red

The BaseScope™ Reagent Kit v2-RED, is based on ACD’s patented signal amplification and background suppression technology. The assay uses a novel and proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in a multitude of sample types mounted on slides. The BaseScope™ Assay is used for the detection of short RNA targets (50-300 bases), highly homologous sequences, RNA mutations, and exon junctions for the detection of splice variants, circular RNA, and gene fusion. The BaseScope™ Assays do not require an RNase-free environment that is used for traditional RNA ISH.